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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
05/02/2015 |
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Data da última atualização: |
01/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CLEMENTE, Z.; CASTRO, V. L. S. S. de; FEITOSA, L. O.; LIMA, R.; JONSSON, C. M.; MAIA, A. de H. N.; FRACETO, L. F. |
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Afiliação: |
ZAIRA CLEMENTE, IB-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; LEANDRO OLIVEIRA FEITOSA, UFSCar; RENATA DE LIMA, Universidade de Sorocaba; CLAUDIO MARTIN JONSSON, CNPMA; ALINE DE HOLANDA NUNES MAIA, CNPMA; LEONARDO FERNANDES FRACETO, UNESP-Sorocaba. |
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Título: |
Biomarker evaluation in fish after prolonged exposure to nano-TiO2: influence of illumination conditions and crystal phase. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Nanoscience and Nanotechnology, Stevenson Ranch, v. 15, n. 7., p. 5424-5433, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In this study, we evaluated the effects of prolonged exposure to two different nano-TiO2 crystal phases under different illumination conditions. Fish (Piaractus mesopotamicus) were exposed for 21 days to 100 mg/L of nano-TiO2 anatase and a mixture of anatase:rutile (80%:20%) under visible light and UV light (UVA and B, 22.47 J/cm2/h). The following oxidative stress biomarkers were monitored:concentrations of lipid hydroperoxide (LPO), carbonylated proteins (PCO), and specific activitiesof superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Otherbiomarkers as well as specific activities of acid phosphatase (AP), Na+, K+-ATPase and metallothioneinlevels (MT) were also evaluated. Moreover, micronucleus and comet assays were performed to assess genotoxicity. Our results showed low toxicity of nano-TiO2 to fish and lack of titanium accumulation in muscle tissue. However, it was observed the occurrence of sublethal effects that were influenced by nano-TiO2 crystal phase and illumination condition. Pure anatase caused more oxidative damage without co-exposure to UV, while the mixture anatase:rutile caused more sublethal effects when exposure occurred under UV. These findings show that the specific activity of CAT, GST, PCO levels and comet assay are useful as biomarkers of prolonged exposure to nano-TiO2. Overall, our study substantiates the development and implementation of nanoecotoxicological protocols. |
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Palavras-Chave: |
Nanopartículas; Nanotoxicity; Titan dioxide; Ultraviolet Light. |
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Thesagro: |
Pacu; Peixe de água doce; Raio ultravioleta; Substância tóxica; Toxidez. |
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Thesaurus Nal: |
Biomarkers; Ecotoxicology; Nanoparticles; Oxidative Stress; Piaractus mesopotamicus; Ultraviolet radiation. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02587naa a2200373 a 4500
001 2007931
005 2023-03-01
008 2015 bl uuuu u00u1 u #d
100 1 $aCLEMENTE, Z.
245 $aBiomarker evaluation in fish after prolonged exposure to nano-TiO2$binfluence of illumination conditions and crystal phase.$h[electronic resource]
260 $c2015
520 $aAbstract: In this study, we evaluated the effects of prolonged exposure to two different nano-TiO2 crystal phases under different illumination conditions. Fish (Piaractus mesopotamicus) were exposed for 21 days to 100 mg/L of nano-TiO2 anatase and a mixture of anatase:rutile (80%:20%) under visible light and UV light (UVA and B, 22.47 J/cm2/h). The following oxidative stress biomarkers were monitored:concentrations of lipid hydroperoxide (LPO), carbonylated proteins (PCO), and specific activitiesof superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Otherbiomarkers as well as specific activities of acid phosphatase (AP), Na+, K+-ATPase and metallothioneinlevels (MT) were also evaluated. Moreover, micronucleus and comet assays were performed to assess genotoxicity. Our results showed low toxicity of nano-TiO2 to fish and lack of titanium accumulation in muscle tissue. However, it was observed the occurrence of sublethal effects that were influenced by nano-TiO2 crystal phase and illumination condition. Pure anatase caused more oxidative damage without co-exposure to UV, while the mixture anatase:rutile caused more sublethal effects when exposure occurred under UV. These findings show that the specific activity of CAT, GST, PCO levels and comet assay are useful as biomarkers of prolonged exposure to nano-TiO2. Overall, our study substantiates the development and implementation of nanoecotoxicological protocols.
650 $aBiomarkers
650 $aEcotoxicology
650 $aNanoparticles
650 $aOxidative Stress
650 $aPiaractus mesopotamicus
650 $aUltraviolet radiation
650 $aPacu
650 $aPeixe de água doce
650 $aRaio ultravioleta
650 $aSubstância tóxica
650 $aToxidez
653 $aNanopartículas
653 $aNanotoxicity
653 $aTitan dioxide
653 $aUltraviolet Light
700 1 $aCASTRO, V. L. S. S. de
700 1 $aFEITOSA, L. O.
700 1 $aLIMA, R.
700 1 $aJONSSON, C. M.
700 1 $aMAIA, A. de H. N.
700 1 $aFRACETO, L. F.
773 $tJournal of Nanoscience and Nanotechnology, Stevenson Ranch$gv. 15, n. 7., p. 5424-5433, 2015.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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Origem |
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Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
26/10/2015 |
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Data da última atualização: |
26/10/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CANOVA, S. P.; REYES, L. F.; MELO, I. S. de. |
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Afiliação: |
S. P. CANOVA, ICB/USP; L. F. REYES, ICB/USP; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Atividade antagônica de actinobactérias isoladas da rizosfera de rhizophora mangle. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 10., 2006, Goiânia, GO. [Anais...]. Goiânia, GO: UFG, 2006. |
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Idioma: |
Português |
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Thesagro: |
Rizosfera. |
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Thesaurus NAL: |
Actinobacteria. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131757/1/2006RA-020.pdf
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Marc: |
LEADER 00521nam a2200145 a 4500
001 2027206
005 2015-10-26
008 2006 bl uuuu u00u1 u #d
100 1 $aCANOVA, S. P.
245 $aAtividade antagônica de actinobactérias isoladas da rizosfera de rhizophora mangle.$h[electronic resource]
260 $aIn: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 10., 2006, Goiânia, GO. [Anais...]. Goiânia, GO: UFG$c2006
650 $aActinobacteria
650 $aRizosfera
700 1 $aREYES, L. F.
700 1 $aMELO, I. S. de
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